Entry STF0015
Northern leopard frog onconase
Protein information
| Name of the protein: | Protein P-30 |
| Organism: | Lithobates pipiens |
| Number of residues: | 104 |
| Related UniProt entry: | P22069 (Fragment: 2 - 104) |
| Related PDB entry: | 1ONC |
Visualize the data
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Experiment sets
EARLY
Method: Quenched-flow HDX NMR
Conditions: pH 5.5; 20.0 Celsius; Probes: 42
Related publication:
PMID 19655705
Experiment details: "Quenched-flow experiments were carried out at 20 °C using a rapid mixing quenched-flow machine. In the first mixing step, refolding of protonated, unfolded ONC (in 6 M GdnHCl) was initiated by a 9-fold dilution into 50 mM sodium acetate buffer, pH 5.5. After defined refolding times (60 - 5000 ms), the samples were diluted 10-fold into D2O buffer (200 mM glycine buffer, 0.667 M GdnHCl, pD 11.0) to initiate the H/D exchange. The exchange was terminated after 8.3 ms by lowering the pH by a 2-fold dilution with D2O, containing 10 M acetic acid and 0.667 M GdnHCl."
Protection threshold: highly protected within 250 ms
Sequence:
EDWLTFQKKHITNTRDVDCDNIMSTNLFHCKDKNTFIYSRPEPVKAICKGIIASKNVLTTSEFYLSDCNVTSRPCKYKLKKSTNKFCVTCENQAPVHFVGVGSC
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EARLY residues
6: F;
7: Q;
8: K;
9: K;
10: H;
11: I;
12: T;
36: F;
37: I;
38: Y;
39: S;
44: V;
45: K;
46: A;
47: I;
48: C;
49: K;
65: L;
66: S;
67: D;
86: F;
87: C;
88: V;
89: T;
90: C;
91: E;
93: Q;
94: A;
96: V;
97: H;
99: V;
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INTERMEDIATE
Method: Quenched-flow HDX NMR
Conditions: pH 5.5; 20.0 Celsius; Probes: 42
Related publication:
PMID 19655705
Experiment details: "Quenched-flow experiments were carried out at 20 °C using a rapid mixing quenched-flow machine. In the first mixing step, refolding of protonated, unfolded ONC (in 6 M GdnHCl) was initiated by a 9-fold dilution into 50 mM sodium acetate buffer, pH 5.5. After defined refolding times (60 - 5000 ms), the samples were diluted 10-fold into D2O buffer (200 mM glycine buffer, 0.667 M GdnHCl, pD 11.0) to initiate the H/D exchange. The exchange was terminated after 8.3 ms by lowering the pH by a 2-fold dilution with D2O, containing 10 M acetic acid and 0.667 M GdnHCl."
Protection threshold: protected after 250 ms
Sequence:
EDWLTFQKKHITNTRDVDCDNIMSTNLFHCKDKNTFIYSRPEPVKAICKGIIASKNVLTTSEFYLSDCNVTSRPCKYKLKKSTNKFCVTCENQAPVHFVGVGSC
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INTERMEDIATE residues
57: V;
59: T;
68: C;
69: N;
70: V;
76: K;
77: Y;
78: K;
80: K;
82: S;
84: N;
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STRONG
Method: Native exchange NMR
Conditions: pH 5.5; 20.0 Celsius; Probes: all
Related publication:
PMID 19655705
Experiment details: "To assess native state H/D exchange in ONC, a series of 2D 15N-HSQC spectra was recorded for 3 days on an Avance II 600 NMR spectrometer after dissolving ONC in D2O buffer."
Protection threshold: amids that persist to exchange for 24 hours
Sequence:
EDWLTFQKKHITNTRDVDCDNIMSTNLFHCKDKNTFIYSRPEPVKAICKGIIASKNVLTTSEFYLSDCNVTSRPCKYKLKKSTNKFCVTCENQAPVHFVGVGSC
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STRONG residues
6: F;
7: Q;
8: K;
9: K;
10: H;
11: I;
36: F;
37: I;
38: Y;
39: S;
44: V;
45: K;
46: A;
47: I;
48: C;
49: K;
57: V;
59: T;
65: L;
66: S;
67: D;
68: C;
69: N;
70: V;
76: K;
77: Y;
78: K;
80: K;
82: S;
84: N;
86: F;
87: C;
88: V;
89: T;
90: C;
91: E;
93: Q;
94: A;
96: V;
97: H;
99: V;
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