Entry STF0027
Ubiquitin
Protein information
| Name of the protein: | Polyubiquitin-C |
| Organism: | Homo sapiens |
| Number of residues: | 76 |
| Related UniProt entry: | P0CG48 (Fragment: 609 - 684) |
| Related PDB entry: | 1UBQ |
Visualize the data
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Experiment sets
EARLY
Method: Pulse labeling HDX NMR
Conditions: pH 5.0; 25.0 Celsius; Probes: 26
Related publication:
PMID 1312711
Experiment details: "Refolding was initiated by rapid 6-fold dilution with 50 mM acetic acid at pH 5.5 in H2O (or D2O for folding times >1 s). The final pH of the mixture was 5.0, as determined in a separate control. The resulting GdnHCI concentration, 1.0 M, is well below the refolding transition. After the desired refolding time, the labeling pulse was initiated by mixing the protein with an equal volume of 100 mM glycine in H2O at pH 9.75, to give a final pH of 9.4. The pulse was terminated after 31 ms by mixing with an equal volume of 500 mM acetic acid at pH 2.6, yielding a final pH of 3.4."
Protection threshold: at least 80% protection in 8 ms
Sequence:
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
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EARLY residues
3: I;
4: F;
5: V;
6: K;
13: I;
16: E;
17: V;
21: D;
23: I;
26: V;
28: A;
29: K;
30: I;
41: Q;
42: R;
44: I;
45: F;
50: L;
55: T;
56: L;
65: S;
67: L;
70: V;
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LATE
Method: Pulse labeling HDX NMR
Conditions: pH 5.0; 25.0 Celsius; Probes: 26
Related publication:
PMID 1312711
Experiment details: "Refolding was initiated by rapid 6-fold dilution with 50 mM acetic acid at pH 5.5 in H2O (or D2O for folding times >1 s). The final pH of the mixture was 5.0, as determined in a separate control. The resulting GdnHCI concentration, 1.0 M, is well below the refolding transition. After the desired refolding time, the labeling pulse was initiated by mixing the protein with an equal volume of 100 mM glycine in H2O at pH 9.75, to give a final pH of 9.4. The pulse was terminated after 31 ms by mixing with an equal volume of 500 mM acetic acid at pH 2.6, yielding a final pH of 3.4."
Protection threshold: 80% protection over 10 ms
Sequence:
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
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LATE residues
59: Y;
61: I;
69: L;
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STRONG
Method: Native exchange NMR
Conditions: pH 3.5; 22.0 Celsius; Probes: all
Related publication:
PMID 1332757
Experiment details: "None"
Protection threshold: amides which persist exchange
Sequence:
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
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STRONG residues
3: I;
4: F;
5: V;
7: T;
17: V;
23: I;
26: V;
28: A;
29: K;
30: I;
44: I;
56: L;
59: Y;
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