Entry STF0035
PDZ-3 from PSD-95
Protein information
| Name of the protein: | Disks large homolog 4 |
| Organism: | Rattus norvegicus |
| Number of residues: | 119 |
| Related UniProt entry: | P31016 (Fragment: 302 - 402) |
| Related PDB entry: | 1BE9 |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 6.0; 25.0 Celsius; Probes: 33
Related publication:
PMID 15663949
Experiment details: "The experiment was done in D2O using 15N-labeled PDZ-3 at pDread 6.0 and 25 °C. The well-dispersed cross-peaks allow the exchange rate constants, k(ex), to be measured accurately for slowly exchanging amide protons."
Protection threshold: ΔG(HX) > 7 kcal/mol
Sequence:
GSPEFLGEEDIPREPRRIVIHRGSTGLGFNIIGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEANSRVNSSGRIVTN
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STRONG residues
18: I;
20: I;
41: F;
42: I;
58: R;
60: G;
61: D;
62: Q;
63: I;
64: L;
65: S;
66: V;
82: A;
92: I;
93: I;
94: A;
95: Q;
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MEDIUM
Method: Native exchange NMR
Conditions: pH 6.0; 25.0 Celsius; Probes: 33
Related publication:
PMID 15663949
Experiment details: "The experiment was done in D2O using 15N-labeled PDZ-3 at pDread 6.0 and 25 °C. The well-dispersed cross-peaks allow the exchange rate constants, k(ex), to be measured accurately for slowly exchanging amide protons."
Protection threshold: 5 < ΔG(HX) (kcal/mol) < 7
Sequence:
GSPEFLGEEDIPREPRRIVIHRGSTGLGFNIIGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEANSRVNSSGRIVTN
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MEDIUM residues
30: N;
32: I;
40: I;
43: S;
69: V;
71: L;
79: A;
80: A;
81: I;
83: L;
84: K;
90: V;
97: K;
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WEAK
Method: Native exchange NMR
Conditions: pH 6.0; 25.0 Celsius; Probes: 33
Related publication:
PMID 15663949
Experiment details: "The experiment was done in D2O using 15N-labeled PDZ-3 at pDread 6.0 and 25 °C. The well-dispersed cross-peaks allow the exchange rate constants, k(ex), to be measured accurately for slowly exchanging amide protons."
Protection threshold: ΔGHX < 5 kcal/mol
Sequence:
GSPEFLGEEDIPREPRRIVIHRGSTGLGFNIIGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEANSRVNSSGRIVTN
CLICK TO DOWNLOAD SEQUENCE IN FASTA
WEAK residues
16: R;
46: L;
91: T;
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