Entry STF0036
Redesigned apo-cytochrome b562
Protein information
| Name of the protein: | Soluble cytochrome b562 |
| Organism: | Escherichia coli |
| Number of residues: | 106 |
| Related UniProt entry: | P0ABE7 (Fragment: 23 - 128) |
| Related PDB entry: | 1YYX |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 7.0; 25.0 Celsius; Probes: 33
Related publication:
PMID 12069590
Experiment details: "The exchange reaction was initiated by dissolving protein samples with deuterated amide protons in an exchanging solution using a spin column filled with G25 Sephadex and preequilibrated with exchanging buffer (50 mM NaAc-d, 95% H2O, and 5% D2O). The deuterated Rd-apocyt b562 protein was prepared by dissolving proteins in D2O (pD 7.0, 0.1 M Na2PO4) and heated at 60 °C for more than 30 min. 15N-1H HSQC spectra were recorded using a 500 MHz instrument. Protein concentrations are 2 mM. The peak intensity as a function of time was measured and fitted to single-exponential kinetics to obtain the exchange rate constant."
Protection threshold: ΔGu =~9.6 kcal/mol
Sequence:
ADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTIRAYNQKYG
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STRONG residues
26: V;
27: K;
28: D;
29: A;
32: K;
35: A;
40: A;
70: G;
71: Q;
75: A;
77: K;
79: A;
80: N;
81: E;
83: K;
87: A;
88: Q;
89: A;
94: L;
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MEDIUM
Method: Native exchange NMR
Conditions: pH 7.0; 25.0 Celsius; Probes: 33
Related publication:
PMID 12069590
Experiment details: "The exchange reaction was initiated by dissolving protein samples with deuterated amide protons in an exchanging solution using a spin column filled with G25 Sephadex and preequilibrated with exchanging buffer (50 mM NaAc-d, 95% H2O, and 5% D2O). The deuterated Rd-apocyt b562 protein was prepared by dissolving proteins in D2O (pD 7.0, 0.1 M Na2PO4) and heated at 60 °C for more than 30 min. 15N-1H HSQC spectra were recorded using a 500 MHz instrument. Protein concentrations are 2 mM. The peak intensity as a function of time was measured and fitted to single-exponential kinetics to obtain the exchange rate constant."
Protection threshold: ΔGu =~6.5 kcal/mol
Sequence:
ADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTIRAYNQKYG
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MEDIUM residues
95: K;
98: I;
99: R;
100: A;
101: Y;
106: G;
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WEAK
Method: Native exchange NMR
Conditions: pH 7.0; 25.0 Celsius; Probes: 33
Related publication:
PMID 12069590
Experiment details: "The exchange reaction was initiated by dissolving protein samples with deuterated amide protons in an exchanging solution using a spin column filled with G25 Sephadex and preequilibrated with exchanging buffer (50 mM NaAc-d, 95% H2O, and 5% D2O). The deuterated Rd-apocyt b562 protein was prepared by dissolving proteins in D2O (pD 7.0, 0.1 M Na2PO4) and heated at 60 °C for more than 30 min. 15N-1H HSQC spectra were recorded using a 500 MHz instrument. Protein concentrations are 2 mM. The peak intensity as a function of time was measured and fitted to single-exponential kinetics to obtain the exchange rate constant."
Protection threshold: ΔGu =~4.5 kcal/mol
Sequence:
ADLEDNWETLNDNLKVIEKADNAAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLANEGKVKEAQAAAEQLKTTIRAYNQKYG
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WEAK residues
8: E;
9: T;
10: L;
14: L;
16: V;
18: E;
65: F;
66: D;
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