Entry STF0055
P. aeruginosa PSE-4 beta-lactamase
Protein information
| Name of the protein: | Beta-lactamase PSE-4 |
| Organism: | Pseudomonas aeruginosa |
| Number of residues: | 271 |
| Related UniProt entry: | P16897 (Fragment: 18 - 288) |
| Related PDB entry: | 1G68 |
Visualize the data
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Experiment sets
STRONG
Method: Native exchange NMR
Conditions: pH 6.65; 25.0 Celsius; Probes: 226
Related publication:
PMID 19486690
Experiment details: "Amide exchange experiments proceeded from TROSY-HSQC spectra of lyophilized PSE-4 dissolved in D2O. The TROSY implementation allowed smaller phase cycling so very short spectra could be acquired with limited baseline distortions. At the beginning, more frequent short spectra (with a low number of transients) were acquired, and at the end, fewer long spectra (with increasing transients) were acquired. To determine the exchange regime, amide exchange experiments were performed for 80 days at pH 6.65, and for six days at pH 7.85. In both situations, 60 spectra were recorded."
Protection threshold: ΔGHX > 8
Sequence:
SSSKFQQVEQDVKAIEVSLSARIGVSVLDTQNGEYWDYNGNQRFPLTSTFKTIACAKLLYDAEQGKVNPNSTVEIKKADLVTYSPVIEKQVGQAITLDDACFATMTTSDNTAANIILSAVGGPKGVTDFLRQIGDKETRLDRIEPDLNEGKLGDLRDTTTPKAIASTLNKFLFGSALSEMNQKKLESWMVNNQVTGNLLRSVLPAGWNIADRSGAGGFGARSITAVVWSEHQAPIIVSIYLAQTQASMEERNDAIVKIGHSIFDVYTSQSR
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STRONG residues
9: E;
12: V;
24: G;
25: V;
26: S;
27: V;
28: L;
38: Y;
55: C;
56: A;
61: D;
62: A;
129: F;
225: A;
226: V;
227: V;
235: I;
236: I;
237: V;
238: S;
239: I;
240: Y;
256: V;
259: G;
260: H;
261: S;
264: D;
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MEDIUM
Method: Native exchange NMR
Conditions: pH 6.65; 25.0 Celsius; Probes: 226
Related publication:
PMID 19486690
Experiment details: "Amide exchange experiments proceeded from TROSY-HSQC spectra of lyophilized PSE-4 dissolved in D2O. The TROSY implementation allowed smaller phase cycling so very short spectra could be acquired with limited baseline distortions. At the beginning, more frequent short spectra (with a low number of transients) were acquired, and at the end, fewer long spectra (with increasing transients) were acquired. To determine the exchange regime, amide exchange experiments were performed for 80 days at pH 6.65, and for six days at pH 7.85. In both situations, 60 spectra were recorded."
Protection threshold: 6 < ΔGHX < 8
Sequence:
SSSKFQQVEQDVKAIEVSLSARIGVSVLDTQNGEYWDYNGNQRFPLTSTFKTIACAKLLYDAEQGKVNPNSTVEIKKADLVTYSPVIEKQVGQAITLDDACFATMTTSDNTAANIILSAVGGPKGVTDFLRQIGDKETRLDRIEPDLNEGKLGDLRDTTTPKAIASTLNKFLFGSALSEMNQKKLESWMVNNQVTGNLLRSVLPAGWNIADRSGAGGFGARSITAVVWSEHQAPIIVSIYLAQTQASMEERNDAIVKIGHSIFDVYTSQSR
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MEDIUM residues
11: D;
13: K;
14: A;
15: I;
17: V;
21: A;
22: R;
23: I;
29: D;
36: W;
42: Q;
44: F;
53: I;
54: A;
57: K;
59: L;
60: Y;
63: E;
98: D;
99: D;
100: A;
101: C;
102: F;
117: L;
118: S;
119: A;
120: V;
122: G;
126: V;
127: T;
128: D;
130: L;
131: R;
132: Q;
133: I;
135: D;
138: T;
139: R;
141: D;
142: R;
144: E;
147: L;
157: D;
158: T;
159: T;
160: T;
164: I;
165: A;
166: S;
168: L;
169: N;
171: F;
172: L;
173: F;
185: L;
186: E;
187: S;
188: W;
189: M;
190: V;
207: W;
208: N;
210: A;
211: D;
223: I;
224: T;
241: L;
242: A;
254: A;
255: I;
257: K;
262: I;
265: V;
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