Pulse labeling HDX NMR None at least 80% protection in 8 ms MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

Refolding was initiated by rapid 6-fold dilution with 50 mM acetic acid at pH 5.5 in H2O (or D2O for folding times >1 s). The final pH of the mixture was 5.0, as determined in a separate control. The resulting GdnHCI concentration, 1.0 M, is well below the refolding transition. After the desired refolding time, the labeling pulse was initiated by mixing the protein with an equal volume of 100 mM glycine in H2O at pH 9.75, to give a final pH of 9.4. The pulse was terminated after 31 ms by mixing with an equal volume of 500 mM acetic acid at pH 2.6, yielding a final pH of 3.4.

Pulse labeling HDX NMR None 80% protection over 10 ms MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

Refolding was initiated by rapid 6-fold dilution with 50 mM acetic acid at pH 5.5 in H2O (or D2O for folding times >1 s). The final pH of the mixture was 5.0, as determined in a separate control. The resulting GdnHCI concentration, 1.0 M, is well below the refolding transition. After the desired refolding time, the labeling pulse was initiated by mixing the protein with an equal volume of 100 mM glycine in H2O at pH 9.75, to give a final pH of 9.4. The pulse was terminated after 31 ms by mixing with an equal volume of 500 mM acetic acid at pH 2.6, yielding a final pH of 3.4.

Native exchange NMR None amides which persist exchange MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

None