Native exchange NMR None 4.0 < log(P) < 4.3 ADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM

The H/D exchange of the peptide N1H groups in both proteins was monitored by recording successive magnitude COSY or HSQC spectra, following the transfer of the protein from H2O to D2O. For the wild-type CheY, by monitoring the H-ex with quickly acquired HSQC spectra on a uniformly 15N-labelled protein sample, it was possible to work at pH 6 despite the faster intrinsic exchange.

Native exchange NMR None 3.3 < log(P) < 4.0 ADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM

The H/D exchange of the peptide N1H groups in both proteins was monitored by recording successive magnitude COSY or HSQC spectra, following the transfer of the protein from H2O to D2O. For the wild-type CheY, by monitoring the H-ex with quickly acquired HSQC spectra on a uniformly 15N-labelled protein sample, it was possible to work at pH 6 despite the faster intrinsic exchange.

Native exchange NMR None log(P) > 4.3 ADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM

The H/D exchange of the peptide N1H groups in both proteins was monitored by recording successive magnitude COSY or HSQC spectra, following the transfer of the protein from H2O to D2O. For the wild-type CheY, by monitoring the H-ex with quickly acquired HSQC spectra on a uniformly 15N-labelled protein sample, it was possible to work at pH 6 despite the faster intrinsic exchange.